AIChE Symposium Series, Volume 88, Issue 290

adsorbed adsorption affinity chromatography Albertsson amino acid ammonium sulfate aqueous two-phase behavior binding Biochem biomolecules bottom phase buffer bulk gel cell chelate Chem Chemical Chromatogr chymotrypsinogen column composite correlation crosslinking density function Dextran diameter distribution effect electro-osmotic flow elution profiles endotoxin equation equilibrium coefficient experimental Figure fraction glycophorin Guiochon half-normal heterogeneity histidine hydrate formation IMAC immobilized increased insulin interactions interfacial ionic strength isoelectric isoelectric point isotherm K₁ Lane ligand ligand molecules liquid log-normal mass transfer matrix method mg/ml micellar micelles molecular mass molecular weight Nafion ovalbumin parameters particle partition coefficient pdf's peak Phys plate numbers polyampholyte polymer Porath precipitation system pressure protease inhibitors protein extract protein partitioning purification recombinant residues resin retention reversed micelles salt concentration sample separation simulation sodium solubility solution solvent soybean protein standard deviation supernatant surface technique top phase two-phase systems ultrafiltration ultrafiltration membranes volume XM300 membrane