A Manual of Bacteriology |
Common terms and phrases
aërobic agar alcohol aniline dyes animals anthrax bacillus appears bacillus coli communis bacterium bichloride of mercury blood blood-serum body bouillon bubonic plague carbolic acid carbon cavity cells cent cholera coagulated colonies color containing cover-glass cultivated culture-media decolorized diphtheria diphtheria bacillus diplococci disease disinfectant flagella flame fluid form spores formation gelatin gelatin plates germicidal glanders gonococcus Gram's method grams grows growth guinea-pigs heat immunity incubator indol infection inoculation intestines large number liquefy gelatin medium micrococci milk minutes motile number of bacteria occur ordinary organisms pathogenic bacteria peptone placed platinum wire plug poisons potato produce pure culture pyogenic bacteria rabbits rapidly reaction septicemia serum skin sometimes species spirilla spirillum spirillum of cholera spores sputum stained by Gram's sterilized streptococcus pyogenes substances suppuration surface temperature teria test-tube tetanus tion tissues toxins tube tubercle tubercle bacilli tuberculosis typhoid bacillus typhoid fever usually virulence xylol
Popular passages
Page 89 - Liquid cultures, or cultures of micro-organisms in media that are fluid at the ordinary temperature (below 45° C., or 113° F.), are unmailable. Such specimens may be sent in media that remain solid at ordinary temperatures. Upon the outside of every package shall be written or printed the words, "Specimen for Bacteriological Examination. This package to be treated as letter mail.
Page 90 - Upon the outside of every package of diseased tissues admitted to the mails shall be written or printed the words, "Specimen for bacteriological examination. This package to be treated as letter mail." No package containing diseased tissues shall be delivered to any representative of any of said laboratories until a permit shall have first been issued by the Postmaster-General, certifying that said institution has been found to be entitled, in accordance with the requirements of this regulation,...
Page 90 - No package containing diseased tissue shall be delivered to any representative of any of said laboratories until a permit shall have first been issued by the...
Page 140 - It may seem paradoxical, but there is truth in the statement that persons rarely die of the disease with which they suffer.
Page 23 - A sufficient amount of the bacteria adherent to the needle are washed off in the drop of water previously placed on the cover-glass and smeared over its surface. The bacteria on the glass are then allowed to dry in the air. The cover-glass is then passed quickly through the flame of a Bunsen burner or alcohol lamp, three times in the usual way, covered with a few drops of Loffler's solution of alkaline methylene-blue, and left without heating for ten minutes.
Page 17 - A layer of thickened oil of cedar-wood is placed between the lower surface of the objective and the upper surface of the glass covering the object under examination. The oil must be wiped away from the surface of the objective when the examination is finished.
Page 59 - I o parts each of common salt, cream of tartar, and acetate of copper, and add a little water ; mix well, and smear the object with it, and then allow it to dry, at the ordinary temperature, from twenty-four to forty-eight hours. At the end of that time the article will be found to be entirely covered with verdigris, which presents various tints.
Page 39 - Hematoxylin crystals 4 grams. Alcohol 25 cc Ammonia alum 50 grams. Water 400 cc Glycerin 100 cc Methyl alcohol 100 cc Dissolve the hematoxylin in the alcohol, and the ammonia alum in the water. Mix the two solutions. Let the mixture stand four or five days uncovered; it should have become a deep purple. Filter and add the glycerin and the methyl alcohol. After it has become dark enough, filter again. Keep it a month or longer before using; the solution improves with age. At the time of using, filter...
Page 25 - ... manifest an affinity for basic dyes (basophile, 8 and y) , others for acid dyes (acidophile or eosinophile, a), and others for a mixture of the two (neutrophile, «), and others still for both the acid and the basic (amphophile, ft). It is best to keep on hand saturated alcoholic solutions of these dyes, from which watery solutions may be made when needed, by adding a few drops of the alcoholic solution to a small dish filled with water. The basic dyes may be used as nuclear stains as follows:...
Page 39 - Distilled water ...... 400 cc. Glycerin ........ 100 cc. Methyl alcohol 100 cc. Dissolve the...